Cell Disruption with High Pressure Homogenizer

 

Cell Disruption with High Pressure Homogenizer

Cell disruption is a crucial process in biotechnology, pharmaceutical research, and life sciences. Efficiently breaking open cells allows researchers to extract valuable intracellular components, including proteins, enzymes, nucleic acids, and viral vectors. One of the most reliable and scalable tools for this purpose is a high pressure homogenizer. NanoGenizer offers advanced solutions that combine precision, reproducibility, and thermal control.

For more on the high pressure microfluidization homogenization technology, see our article: High Pressure Homogenizer Introduction
 

How NanoGenizer High Pressure Homogenizer Achieves Cell Disruption

The NanoGenizer high pressure homogenizer operates by forcing a cell suspension through diamond interaction chambers under high pressure. This generates controlled shear, impact, and cavitation forces that rupture cell membranes while preserving delicate intracellular contents. For heat-sensitive samples, RT chambers with cooling systems prevent protein and nucleic acid denaturation, ensuring consistent quality across multiple batches.

NanoGenizer microfluidic jet high pressure homogenizer for cell disruption

Figure 1. NanoGenizer microfluidic jet high pressure homogenizer


Optimizing Pressure and Chamber Selection for Efficient Cell Disruption

Different biological cells require different pressures to achieve effective lysis. Selecting the appropriate pressure and chamber ensures high disruption efficiency while protecting sensitive intracellular components.
 

Pressure and chamber selection for various applications with NanoGenizer

CellsMammalian cell
Mammalian cell		Insect cell
Insect cell		Bacteria cell
Bacteria cell		Yeast cell
Yeast cell		Algae cell
Algae cell		Spore
SPore Ganoderma
SampleCHO|HelaSf9|Sf21|H5E. Coli  YeastGreen algaeGanoderma
Pressure600-800bar
8500-11500psi		600-1000bar
8500-14500psi		800-1000bar
11500-14500psi		1200-1400bar
17500-20000psi		1200-1500bar
7500-21500psi		1300-1600bar
18500-23000psi
ChamberF20Y, F20Y-RTF20Y, F20Y-RTF20Y, F20Y-RTF20Y, F12YF20Y, F12YF20Y, F12Y

 

  • Softer cells (mammalian, insect) require lower shear.

  • Bacteria have stronger cell walls and need higher pressure.

  • Yeast, algae, and spores require the highest pressure due to rigid structures.

Using the NanoGenizer’s microfluidic diamond interaction chamber, researchers can achieve consistent, efficient cell disruption while maintaining protein and nucleic acid integrity.

Internal Structure of Z-type Interaction Chambers for High Pressure Homogenizers

Figure 2. Internal Structure of Z-type Interaction Chambers for High Pressure Homogenizers


Advantages of High Pressure Homogenizer for Cell Disruption

Compared to other mechanical or chemical methods, high pressure homogenizers like NanoGenizer offer:

  • High efficiency: Rapidly breaks even tough cell walls with fewer passes.

  • Thermal control: Cooling systems maintain protein and nucleic acid stability.

  • Scalability: Suitable for laboratory R&D and large-scale production.

  • Multi-functionality: Can also resize liposomes, create nanoparticles, or form nanoemulsions (Liposome Extruder).

  • Reproducibility: Ensures uniform results across multiple batches.

Unlike traditional homogenizers that mainly emulsify liquids, NanoGenizer provides precise and consistent cell disruption for a variety of applications.

 

Applications of High Pressure Homogenizer Cell Disruption

High pressure homogenizers are widely used in:

  • Protein and enzyme extraction

  • Vaccine and viral vector development

  • Microbial and yeast fermentation studies

  • Algal bioactive compound extraction

  • Gene therapy research

Optimized operation parameters allow researchers to efficiently release intracellular materials while maintaining functional integrity.
 


For laboratories or biopharmaceutical facilities seeking reliable, scalable, and precise cell disruption solutions, NanoGenizer high pressure homogenizers provide advanced interaction chambers, adjustable pressure, and thermal management. Contact us at info@genizer.com or send an inquiry here to learn how high pressure homogenizers can enhance your research and production workflows.
 

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